Macrowine 2021
IVES 9 IVES Conference Series 9 Metabolomic profile of red non-V. vinifera genotypes

Metabolomic profile of red non-V. vinifera genotypes

Abstract

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera. For this reason, these genotypes have been used in breeding programs in order to introgress resistant traits to pests and diseases in V. vinifera species. The aim of this work was to study the metabolite profiles (simple phenolic compounds, anthocyanins, lipids and proanthocyanidins) of two hybrid varieties (41B and K5BB) and seven American Vitis species (V. andersonii, V. arizonica, V. champinii, V. cinerea, V. riparia, V. slavinii and V. californica) in six different vintages. The results were compared with two V. vinifera cultivars (Pinot Noir and Cabernet Sauvignon). Grape skin anthocyanins were analyzed by HPLC-DAD [1] and twenty different anthocyanins were detected and quantified. In four genotypes less than 5% of the total amount of anthocyanins detected were diglucosides (from 11.6 to 56.9 mg/kg). In the five remaining genotypes more than 50% of the total were found to be diglucosides (from 522.1 to 2657.6 mg/kg). Analysis of phenolic compounds by UHPLC-MS/MS [2] showed that three non-V. vinifera genotypes contained higher average amount of total phenolics compared to V. vinifera cultivars. A rapid LC-MS/MS method [3] was used to identify and quantify thirty-three lipids. The total lipids of eight out of nine non-V. vinifera genotypes was higher compared to V. vinifera cultivars. The differences between the genotypes’ content of anthocyanins, phenolics and lipids were investigated by heatmap analysis. The data was scaled to unit variance, the correlation of variables was used as the distance measure and Ward’s minimum variance method was used for hierarchical clustering. Up to our knowledge this is the most extended metabolomics profiling study on wild Vitis grape genotypes. Altogether, this study highlights the presence of a significant genotypic diversity between the composition of the fruits of V. vinifera and other species. The knowledge of their composition can greatly influence the further breeding programs, since being responsible for both the quality and the resistance traits of new grape interspecific varieties.

[1] Mattivi F. et al. JAFC 2006, 54, 7692-7702 [2] Vrhovsek U. et al. JAFC 2012, 60, 8831-8840 [3] Della Corte A. et al. Talanta 2015, 140, 52-61

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Silvia Ruocco*, Daniele Perenzoni, Fulvio Mattivi, Jan Stanstrup, Marco Stefanini, Urska Vrhovsek

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Sensory and nephelometric analysis of tannin fractions obtained by ultrafiltration of red wines

The assessment of red wine mouthfeel relies primarily on the sensory description of its tannic properties. This evaluation could be improved by gaining a better understanding of the physicochemical properties of these tannins. Hence, the objectives of the present study were threefold: (1) to gain an insight into the sensory properties of subpopulations of proanthocyanidic tannins of different molecular sizes obtained through several ultrafiltration steps, (2) to quantify the kinetics of haze formation of these proanthocyanidic tannins in a dynamic polyvinylpyrrolidone (PVP) precipitation test, (3) to determine whether a correlation exists between the sensory and the precipitation data.

What about oxygen transfer during wine aging in barrels?

During wine aging, several complex phenomena of gas transfer take place in barrels due to the wine/oak contact. The efficiency of this gas transfer varies according to oak wood’s intrinsic physical properties. This research aims to better understand oxygen transfer phenomena through dry oak staves and especially through stave gaps, in order to reevaluate the importance of barrel-making on a barrel’s supply of oxygen. Experimentation was based on the development of an innovative permeameter of laboratory scale, for which the principal operating conditions concerning applied pressure, the choice of liquid phase/gas phase, and the grain type of oak are taken into account and investigated. With a specially developed tightening system, the existing pressure at stave gaps in a barrel could be reproduced on a laboratory scale in order to estimate its influence on oxygen transfer efficiency.

Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Oak barrels form an integral part of wine production, especially that of high quality wines. However, due to its porosity, wood presents an ecological niche for microbial proliferation and is highly susceptible to microbial spoilage which could cause considerable economic losses. Brettanomyces bruxellensis, the most commonly encountered microorganism responsible for spoilage during barrel ageing, can remain in barrels after barrel sanitation to contaminate new batches of wine after refilling. Therefore, effective sanitation treatments are of utmost importance to prevent recurring wine spoilage.

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.

Elicitors used as a tool to increase stilbenes in grapes and wines

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001).