terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

Abstract

Mycobiota encountered from vine to wine is a complex and diversified ecosystem that may impact grape quality at harvest and the sensorial properties of wines, thus leading to off-flavors [1-3]. Among known off-flavors in wine, fresh mushroom aroma (FMA) has been linked to some mold species, naturally pre-sent on grapes, producing specific volatile organic compounds (VOC) [4-5]. The most well-known are 1-octen-3-ol and 1-octen-3-one, although many other VOC are likely involved. To better understand the FMA defect, biotic and abiotic factors impacting growth kinetics and VOC production of selected fungal species in must media and on grapes were studied. In total, 18 fungal isolates, belonging to 7 genera and 9 species, were selected based on their off-odor production profiles on malt medium among 685 isolates from our working collection. Growth rates were measured using solid synthetic must (MS) and real must (MR) and compared to those obtained in liquid must by laser nephelometry. Sensorial analysis and VOC profiles (GC-MS) were also determined for the same isolates, individually or in co-cultures with two FMA producing Botrytis isolates, after growth on must and grapes. Among the generated physiological data, optimal growth temperatures were 27-28°C, 26-30°C, 21-22°C for Botrytis spp., Penicillium crocicola and P. citreonigrum, respectively, depending on the isolate. Fastest growth rates were observed for B. cinerea and P. crocicola, while Cladosporium subtilissimum and P. brevicompactum isolates were slowest. For VOC profiles, P. crocicola, P. bialowiezense and Clonostachys rosea produced known FMA compounds (1-octen-3-one and 1-octen-3-ol) at higher levels when co-inoculated with Botrytis spp. on grapes. For must trials, a species effect on VOC profiles was clearly observed (92 VOC identified). To confirm these findings, further co-inoculation studies were performed on two grape varieties (Meunier and Pinot noir) and, so far, sensorial analyses showed similar trends. Overall, this study provides novel knowledge about changes in fungal growth kinetics and VOC profiles in musts and on grapes. These results provide new insights for the wine making to better understand how FMA off-flavors are generated by molds.

 

1. Scott et al, 2022 doi.org/10.1016/B978-0-08-102067-8.00006-3
2. Steel et al, 2013 doi.org/10.1021/jf400641r
3. Rousseaux et al 2014 doi:10.1016/j.fm.2013.08.013
4. La Guerche et al, 2006 doi.org/10.1016/B978-0-08-102067-8.00006-3
5. Meistermann et al, 2020 (DOI:10.20870/oeno-one.2021.55.3.3004)

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Adrien Destanque1,2, Alexis Commereuc1, Flora Pensec1, Adeline Picot1, Anne Thierry3, Marie-Bernadette Maillard3, Louis Corol-ler, Sylvie Treguer-Fernandez1, Emmanuel Coton1, Marion Hervé2 and Monika Coton1*

1. Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, F-29280 Plouzané, France
2. Centre de Recherche Robert-Jean de Vogüé Moët Hennessy, F-51530 Oiry, France
3. INRAE, Institut Agro, UMR STLO, F-35000 Rennes, France

Contact the author*

Keywords

Mycobiot, growth modeling, volatile organic compounds (VOCs)

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

OPTIMIZING THE IDENTIFICATION OF NEW THIOLS AT TRACE LEVEL IN AGED RED WINES USING NEW OAK WOOD FUNCTIONALISATION STRATEGY

During bottle aging, many thiol compounds are involved in the expression of bouquet of great aged red wines according to the quality of the closure.1,2 Identifying thiol compounds in red wines is a challenging task due several drawbacks including, the complexity of the matrix, the low concentration of these impact compounds and the amount of wine needed.3,4
This work aims to develop a new strategy based on the functionalisation of oak wood organic extracts with H₂S, to produce new thiols, in order to mimic what can happen in red wine during bottle aging. Following this approach and through sensory analysis experiments, we demonstrated that the vanilla-like aroma of fresh oak wood was transformed into intense “meaty” nuances similar to those found in old but non oxidized red wines.

HYDROXYTYROSOL PRODUCTION BY DIFFERENT YEAST STRAINS: SACCHAROMYCES AND NON-SACCHAROMYCES AND THE RELATION WITH THE NITROGEN CONSUMPTION

Hydroxytyrosol (HT) is a phenolic compound with extensive bioactive properties. It is present in olives, olive oil and wines. Its occurrence in wines is partly due to yeast synthetise tyrosol from tyrosine by the Ehrlich pathway, which is subsequently hydroxylated to .
The aim of the present work is to study how different yeast strains can influence in the HT production and, how the different nitrogen consumption of each strain can interfere the production of bioactive compounds.

CHARACTERIZATION OF THE AROMA PROFILE OF COMMERCIAL PROSECCO SPARKLING WINES

The typicality of a wine, as well as its aromatic identity, are attributes that are highly sought after and requested by the current market. It is therefore of considerable technological interest to investigate the aromatic aspects of specific wines and to identify the odorous substances involved.In this thesis work, the characterization of the aromatic composition of Prosecco wines available on the market with a price range between 7 and 13 euros was carried out. These wines came from three different areas of origin such as Valdobbiadene, Asolo and Treviso.

WHICH TERROIR-RELATED FACTORS INFLUENCE THE MOST VOLATILE COMPOUND PRODUCTION IN COGNAC BASE WINE?

Cognac is a famous spirit produced in southwest France in the region of the eponymous town from wines mainly from Vitis vinifera cv. Ugni blanc. This variety gives very acidic and poorly aromatic base wines for distillation which are produced according to a very specific procedure. Grapes are picked at low sugar concentrations ranging 13-21 °Brix and musts with high turbidity (>500 NTU) are fermented without sulphite addition [1]. Fermentative aromas, as esters and higher alcohols, are currently the main quality markers considered in Cognac spirits.

IMPACT OF METSCHNIKOWIA PULCHERRIMA DURING FERMENTATION ON AROMATIC PROFILE OF VIDAL BLANC ICEWINE

Non-Saccharomyces yeasts not only increase microbial diversity during wine fermentation, but also have a positive effect on improving wine aroma. Among these non-Saccharomyces yeast species, Metschnikowia pulcherrima is often studied and used in winemaking in recent years, but its application in icewine has been rarely reported. In this study, indigenous M. pulcherrima strains and Saccharomyces cerevisiae strains (commercial and indigenous strains) were sequentially inoculated for icewine fermentations; meanwhile, pure S. cerevisiae fermentations were used as the control; indigenous strains used above were screened from spontaneous fermentations of Vidal blanc icewine.